產品詳情
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Minute? 哺乳動物細胞/組織線粒體分離試劑盒
類型: 生物試劑
品牌: 英文特
規格: 50 Tests/4 Tests
編號: MP-007
產品詳情
應用


提取出的線粒體可以應用于SDS-PAGE,immunoblottings,ELISA,IP,膜蛋白結構分析,2-D,酶活性檢測和其他應用

Figure 1. Enrichment of Mitochondrial Marker (Ubiquinol-Cytochrome C Reductase Core Protein) with MinuteTM Mitochondria Isolation Kit.
A. SDS-PAGE profiles of total protein extract vs. isolated mitochondrial proteins. Lanes 1, 3 5, total proteins from isolated mouse kidney cells, mouse skeletal muscle and liver tissues respectively. Lanes 2, 4, 6, isolated mitochondrial proteins from mouse kidney cells, mouse skeletal muscle and liver tissues respectively.
B. Western blotting. Proteins in A were transferred to nitrocellulose membranes and probed with anti-ubiquinol-cytochrome C reductase core protein (ab96333, abcam, Cambridge, MA) and anti-lamin B1 (ab16048,a nuclear envelope marker protein. abcam, Cambridge, MA).
C. Densitometry measurement of mitochondrial marker signals.


產品訂購信息
Minute TM Mitochondrial Membrane Protein Isolation Kit (50 Tests) MP-0074327.00
Minute TM Mitochondrial Membrane Protein Isolation Kit (4 Tests)

MP-007

436.00
產品特點


簡單,快速,性價比高

得率高,可獲得更完整的線粒體

處理樣品范圍更廣

緩沖液系統不含表面活性劑和EDTA


參考文獻


1. Gou, R. et al. (2014). Resveratrol suppresses oxidized low-density lipoprotein-induced macrophage apoptosis through inhibition of intracellular reactive oxygen species generation, lox-1 and the p38 MAPK pathway. Cellular Physiology and Biochemistry. 34:603-616. DOI: 10.1159/000363026

2. Hongli Hu, et al (2016).Acetylationof PGK1 Promotes Liver Cancer Cell Proliferation and Tumorigenesis. Hepatology.doi:10.1002/hep.28887


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